Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells
Kuroda T., Yasuda S., Matsuyama S., Tano K., Kusakawa S., Sawa Y., Kawamata S., Sato Y. Regenerative Therapy, 2015
Human iPSCs were maintained on laminin-521 in Essential 8 medium. Cardiac differentiation on laminin-521 and Matrigel. Adult human cardiomyocytes (Promocell, Heidelberg) were cultured on laminin-211 in the Promocell myocyte growth medium. The authors have established a sensitive assay for the detection of the residual undifferentiated hiPSCs in cardiomyocytes, using droplet digital PCR (ddPCR). LIN28 was the most sensitive marker of residual undifferentiated cells in hiPSC-derived cardiomyocytes but also in other tissues such as liver, heart, pancreas, kidney, spinal cord, corneal epithelium, and lung. Hence, the LIN28/ddPCR method is applicable to the quality control of hiPSC-derived cell therapy products.
Talk to our team to get a custom proposal
We are here to help you in your journey.