Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

In this review, the authors look at different large-scale hPSC culture growth components by comparing cell culture methods (matrices, media, etc.) and identifying the advantage and disadvantages and pitfalls associated with each one. Since laminin-521 was not available when the review was written, they only mention laminin-511 where the cells are seeded as clumps (Rodin et al 2010). The only disadvantage mentioned regarding laminin-511 is that the use of laminin for maintenance and expansion will be to thigh due to the price of the laminins.