Improved erythroid differentiation of multiple human pluripotent stem cell lines in microcarrier culture by modulation of Wnt/β-Catenin signaling
Jaichandran Sivalingam, Hong Yu Chen, Bin-Xia Yang, Zhong Ri Lim, Alan Tin Lun Lam, Tsung Liang Woo, Allen Kuan-Liang Chen, Shaul Reuveny, Yuin-Han Loh, Steve Kah-Weng Oh.
This article describes means to scale up the pluripotent expansion stage by culturing hiPSCs on Biolaminin 521 (LN521)-coated microcarriers (MCs). The protocol allows hiPSC-MC aggregates to efficiently differentiate differentiate as embryoid bodies (EBs) in a scalable manner in suspension culture.
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