In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors
Yap L., Wang J.W., Moreno-Moral A., de Kleijn D.P.V., Petretto E., Tryggvason K.
Cell Reports, 2019
This article presents a highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol for generating cardiovascular progenitors (CVPs) from stem cells. Laminin-221 (LN221) was identified as abundantly present in heart tissue and utilized in further studies. The full-length human Biolaminin 221 enhanced differentiation of pluripotent human embryonic stem cells (hESCs) towards the cardiomyocyte lineage while downregulating pluripotency and teratoma-associated genes. CVPs improved heart function after transplantation into myocardial infarction mice, as assessed by echocardiogram. This project aims to produce clinical-quality cells for regenerative cardiology applications.
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