Laminin α5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro

Anu Hyysalo, Mervi Ristola, Meeri E.-L. Mäkinen, Sergei Häyrynen, Matti Nykter, Susanna Narkilahti.

Stem Cell Research, 2017

In this work, the authors compared different recombinant human laminin isoforms in the culture of hPSC derived neurons. They show that LN α5 chain is important for neuronal attachment, viability and network formation, and that neuronal functional development is enhanced by LN α5-substrates.