Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells

Takayama K., Mitani S., Nagamoto Y., Sakurai F., Tachibana M., Taniguchi Y., Sekiguchi K., Mizuguchi H.

Biochem Biophys Res Commun. 2016

Here the authors searched for a suitable extracellular matrix to promote cholangiocyte differentiation from human iPS cells and found that both laminin 411 and laminin 511 were suitable for this purpose. Differentiated human iPSC to cholangiocyte-like cells via hepatoblasts-like cells (derived according to previous papers). The hepatoblast-like cells were mixed in a collagen gel and plated and for cholangiocyte differentiation, laminin was added to the medium. The gene expression levels of the cholangiocyte markers, aquaporin 1 (AQP1), SRY-box 9 (SOX9), cystic fibrosis transmembrane conductance regulator (CFTR), G protein-coupled bile acid receptor 1 (GPBAR1), Jagged 1 (JAG1), secretin receptor (SCTR), and g-glutamyl transferase (GGT1) were increased by using laminin 411 or laminin 511 as a matrix. In addition, the percentage of AQP1-positive cells was increased from 61.8% to 92.5% by using laminin 411 or laminin 511. Furthermore, the diameter and number of cysts consisted of cholangiocyte-like cells were increased when using either matrix.

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