Laminin 411 mediates endothelial specification via multiple signaling axes that converge on β-catenin

The authors show that laminin-411 (Biolaminin 411) alone induces endothelial cell differentiation in human and mouse induced pluripotent stem cells over collagen I or Matrigel. Overall, the results indicate that the extracellular matrix contributes to endothelial differentiation through multiple avenues which converge at β-catenin. This protocol is an improvement to previous methods, excluding the use of undefined matrix substances and being less complex in terms of added small-molecule reagents.