Laminin-511 but not -332, -111, or -411 enables mouse embryonic stem cell self-renewal in vitro

Different laminin isoforms, LN-511, -332, -411 and -111, as well as Matrigel, gelatin and poly-D-lysine are compared as substrates maintaining pluripotent mouse ES cells in vitro without the addition of leukemia inhibitory factor. Conclusions are that only LN-511 is able to sustain self-renewal for up to 169 days of culturing and cells maintain expression of pluripotency markers and can be used for generation of chimeric mice.