Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors

Human fibroblasts were reprogrammed and the resulting iPSC colonies stabilized and expanded under xeno-free conditions by Stemiotics. Colonies were bulk passaged from the most productive well to establish passage 1 (P1) iPSC cultures on Laminin-521 in Nutristem XF media and expanded in the same culture system until at least passage 3 before being frozen down for storage. The iPSC were then used for neural differentiation.