Regulation of Proliferation and Chondrogenic Differentiation of Human Mesenchymal Stem Cells by Laminin-5 (Laminin-332)

Regulation of Proliferation and Chondrogenic Differentiation of Human Mesenchymal Stem Cells by Laminin-5 (Laminin-332)

Hashimoto J., Hashimoto U., Kariya Y., Miyazaki K.
STEM CELLS, 2006

In this study, the authors examined a possible role of laminin 332 in the proliferation and differentiation of human MSCs. When MSCs were incubated in the presence of a coated or soluble form of laminin 332 in a growth medium, they proliferated more rapidly than nontreated cells, keeping their differentiation potential. On the other hand, laminin 332 potently suppressed the chondrogenic differentiation of MSCs. These activities were mediated mainly by integrin a3B1. However, laminin-5 had no effect on the osteogenic differentiation of MSCs. These results suggest that laminin-5 may contribute to the development of bone tissues by promoting proliferation and by suppressing the chondrogenic differentiation of MSCs.