Scientific Spotlight
The easiest path to stable cardiomyocyte differentiation
Learn how Dr. Lynn Yap and colleagues at Duke NUS Singapore achieved highly pure and functional PSC-derived cardiomyocytes with enhanced reproducibility using heart-abundant full-length laminin proteins, Biolaminin 221 and 521.
Consistent cardiomyocyte differentiation across batches and cell lines
Differentiation protocol: Yap et al. 2019 Cell Reports
Enhanced reproducibility
The fastest process and lowest variability on the Biolaminin 221+521 matrix
Using the combination of Biolaminin 221 (full-length laminin-221) and Biolaminin 521 (full-length laminin-521) not only accelerates differentiation to highly pure, contracting cardiomyocytes but also minimizes the variability in the time required to achieve this.
Read more: Barnes et al. 2022 Bioengineering (Basel)
Highly consistent transcriptome profiles during differentiation
A) Consistent gene expression profiles between two different pluripotent stem cell lines during differentiation towards contracting cardiomyocytes, measured by bulk RNA-sequencing. B) High similarity in gene expression levels in progenitor cell populations (CVPs) between processes started in two different stem cell lines, measured by single-cell RNA-sequencing. Each dot represents a gene. Adapted from Yap et al. 2019)
Consistent expression of mature cardiomyocyte markers
Similar expression levels of mature cardiomyocyte markers were measured by bulk RNA-sequencing from two starting cell lines, showing reproducibility across the differentiation process during 94 days.
Read more: Yap et al. 2019 Cell Reports
How did they do it? Hear the story from the lead author
Hear the story from the lead author, Dr. Lynn Yap
The effortless way to efficient differentiation — keep your protocol, just switch to Biolaminin® matrix
Biolaminin substrates work independently of media or cell lines and on various materials, seamlessly integrating with your current protocol.
Intrigued? Explore all the benefits of Biolaminin for your cardiomyocyte culture in our Application note.
The full-length laminin-based method produces pure functional cardiomyocytes that improve heart function in myocardial infarction models with an enhanced safety profile
Dr. Lynn Yap and colleagues have published pre-clinical evidence for their promising myocardial infarction treatment using stem cell-derived committed cardiac progenitors (CCPs). The hESC-CCPs differentiated on Biolaminin 521 and Biolaminin 221 matrix mature and improve heart function in animal models post-transplantation.
Their studies highlight enhanced left ventricular ejection fraction, improved ventricular wall thickness, and reduced infarction size. Importantly, ventricular arrhythmia risk halved and all pigs survived, marking a notable advancement in this field.
Read more in these publications
Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.
Yap et al. 2023 npj Regenerative Medicine
Spatiotemporal Transcriptomes of Pig Hearts Reveal Midkine-Mediated Vascularization in a Chronic Myocardial Infarcted Model.
Adusumalli et al. 2023 BiorXiv
In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors.
Yap et al. 2019 Cell Reports
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