Organotypic arrangement of mouse embryonic lung cells on a basement membrane extract: involvement of laminin

L Schuger, KS O’Shea, BB Nelson, J Varani.

Development, 1990

In this article, the authors cultured single cell suspensions generated by trypsinization of lungs removed from day 12 embryos. The results strongly supports a role for the basement membrane in the organotypic rearrangement of embryonic lung cells and subsequent in vitro cyst formation and budding of the reestablished epithelium.