Single-cell cloning and expansion of human-induced pluripotent stem cells by a microfluidic culture device

Matsumura T., Tatsumi K., Noda Y., Nakanishi N., Okonogi A., Hirano K., Li L., Osumi T., Tada T., Kotera H. 

BBRC, 2014

Validation of increased survival of single-cell hiPSC clones on LN-521 in culture and a microfluidic chip. Here the authors describe increased survival and propagation of single hiPSC clones on LN-521 in both cultures and on a new microfluidic device. By conditioning the medium they show drastically increased efficiency and represent an additional protocol to the LN-521/E-cadherin method presented in Rodin et al., 2014 for clonal growth.