Single-cell cloning and expansion of human-induced pluripotent stem cells by a microfluidic culture device
Matsumura T., Tatsumi K., Noda Y., Nakanishi N., Okonogi A., Hirano K., Li L., Osumi T., Tada T., Kotera H.
Validation of increased survival of single-cell hiPSC clones on LN-521 in culture and a microfluidic chip. Here the authors describe increased survival and propagation of single hiPSC clones on LN-521 in both cultures and on a new microfluidic device. By conditioning the medium they show drastically increased efficiency and represent an additional protocol to the LN-521/E-cadherin method presented in Rodin et al., 2014 for clonal growth.
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