Synthesis and characterization of well-defined hydrogel matrices and their application to intestinal stem cell and organoid culture

In this work, the authors describe a protocol for the generation of well-defined matrices for the culture of intestinal stem cells (ISCs) and intestinal organoids, using PEG hydrogel backbone functionalized with minimal adhesion cues including RGD (Arg-Gly-Asp), which is sufficient for ISC expansion, and laminin-111, which is required for organoid formation.