The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines

Canham M.A, Van Deusen A., Brison D.R., De Sousa P.A., Downie J., Devito L., Hewitt Z.A., Ilic D., Kimber S.J., Moore H.D., Murray H., Kunath T.Scientific Reports, 2015

It is essential to know the genetic stability of the hESC lines before progressing to clinical trials. In this study, they evaluated the molecular karyotype of 25 clinical-grade hESC lines by whole-genome single nucleotide polymorphism (SNP) array analysis. A total of 15 unique copy number variations (CNVs) greater than 100 kb were detected, most of which were found to be naturally occurring in the human population and none were associated with culture adaptation. The hESC lines were all cultured on laminin-521.