Application note

Animal-origin-free and defined keratinocyte culture

Discover how Biolaminin® 521 and 511 substrates enable efficient, animal-origin-free culture of human keratinocytes in defined conditions. These full-length laminins replicate the native basement membrane of the epidermis, promoting robust cell adhesion, proliferation, and differentiation. The system supports reproducible expansion of primary and stem cell–derived keratinocytes with preserved morphology and barrier-forming capacity, offering a physiologically relevant platform for skin research and regenerative applications. 

What’s inside

  • Overview of laminin 521 and 511 in the native skin basement membrane 
  • Defined, animal-origin-free culture of primary and stem cell–derived keratinocytes 
  • Improved attachment, proliferation, and differentiation in vitro 
  • Maintenance of morphology and epidermal barrier function 
  • Applications in skin biology, disease modeling, and regenerative medicine 
Download