Application note

Neural crest differentiation

Discover how Biolaminin® 521 provides a defined, animal-origin-free environment that promotes efficient and reproducible neural crest differentiation from human pluripotent stem cells (hPSCs). The full-length laminin 521 substrate mimics the developmental microenvironment of the neural plate border, supporting cell attachment, survival, and migration while guiding neural crest specification and downstream lineage commitment. 

What’s inside

  • Role of laminin 521 in neural crest induction and development 
  • Defined, animal-origin-free differentiation of hPSCs into neural crest cells 
  • Improved cell attachment, migration, and lineage commitment 
  • Generation of functional neural derivatives such as neurons, glia, and smooth muscle 
  • Reproducible and scalable workflow for developmental and translational studies 
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