Application note

Human ES and iPS cell culture

Full-length, human recombinant Biolaminin 521 (CT521) provides a defined, animal-origin–free substrate that supports stable, feeder-free culture of human pluripotent stem cells (hPSCs) — ideal for clinical research, cell therapy development, and scalable manufacturing.

What’s inside

  • Overview of CT-grade Biolaminin 521 and its compliance with regulatory guidance (animal-origin free, suitable for cell/gene/tissue-engineered products)
  • Evidence for robust, long-term expansion of hPSCs (both human embryonic and induced pluripotent stem cells) as single cells or colonies — without feeder cells or spontaneous differentiation
  • Maintenance of pluripotency, genetic stability (normal karyotype), and differentiation potential (three-germ-layer capacity) over multiple passages on CT521
  • Practical advantages: consistent performance, batch-to-batch quality (Certificate of Analysis, Animal-Origin Free Statement), and ease of use for research and non-commercial manufacturing workflows 
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