Application note

Pluripotent stem cell culture

Discover how Biolaminin® 521 enables consistent, xeno-free expansion and long-term maintenance of human pluripotent stem cells (hPSCs). This full-length laminin substrate supports robust cell attachment, growth, and survival in chemically defined systems — providing a biologically relevant environment that mimics the early embryonic niche and promotes stable self-renewal without spontaneous differentiation. 

What’s inside

  • Overview of Biolaminin® 521 as a full-length laminin for pluripotent stem cell culture 
  • Defined, xeno-free expansion of hESCs and hiPSCs with high reproducibility 
  • Improved attachment, survival, and single-cell passaging efficiency 
  • Maintenance of pluripotency and genomic stability over long-term culture 
  • Seamless integration into clinical-grade, feeder-free workflows 
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