Application note

Hepatocyte differentiation and culture in 2D and 3D

Discover how full-length Biolaminin® substrates support efficient differentiation and long-term culture of hepatocytes in both 2D and 3D systems. Biolaminin 521 and 111 recreate essential liver basement membrane components, promoting cell polarity, maturation, and metabolic activity of PSC-derived hepatocytes and primary liver cells. The defined, xeno-free system enhances reproducibility and functionality for disease modeling, drug screening, and regenerative applications. 

What’s inside

  • Overview of laminins in liver development and hepatocyte niche 
  • Differentiation of PSCs to functional hepatocytes using Biolaminin® 521 and 111 
  • Improved cell morphology, polarity, and metabolic performance in vitro 
  • Application in 2D and 3D hepatic culture systems 
  • Xeno-free and defined conditions for translational liver research 
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