Application note

RPE differentiation

Full-length, human recombinant Biolaminin 521 (LN521) provides a defined, animal-origin–free substrate that supports efficient generation of retinal pigment epithelial (RPE) cells from human pluripotent stem cells — yielding cells with native RPE features and suitability for research or clinical applications

What’s inside

  • Defined LN521-based protocol for deriving RPE from hESCs under clinically compliant, feeder-free conditions
  • High seeding efficiency, cell migration, and formation of uniform RPE monolayers
  • RPE cells showing native morphology, pigmentation, polarization, marker expression, and phagocytic activity
  • Evidence that derived RPE cells integrate in vivo, survive long-term, and rescue photoreceptor function post-transplantation
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