Guidelines

Transfer from: LN521 to MX521 or CT521

This guide outlines how to transfer PSC cultures from LN521 to MX521 or CT521 with minimal changes to existing workflows. It provides practical recommendations to ensure a smooth transition while maintaining cell attachment, morphology, and pluripotency.

What’s inside

• Recommended coating concentrations before, during, and after transition, including guidance on when to increase or decrease coating levels
• Key indicators of cell behavior (e.g. attachment, morphology, colony formation) to monitor and optimize the transfer process
• Practical compatibility guidance covering media, passaging methods, and ROCK inhibitor use, ensuring stable culture conditions across substrates