Instructions

Neural Crest differentiation from hPSCs

This instruction describes a defined, feeder-free method for differentiating human pluripotent stem cells (hPSCs) into neural-crest cells using Biolaminin 521 as the culture substrate, supporting reproducible lineage induction and xeno-free workflows.

What’s inside

  • Coating steps for preparing Biolaminin 521 plates
  • Simple day-by-day neural-crest differentiation workflow
  • Guidance for handling and passaging cells during induction
  • Expected outcome: neural-crest cells in ~5–7 days with typical NC markers
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