Instructions

Biosilk and Biolaminin® 3D microfiber networks

Biosilk is a fully defined recombinant material that self-assembles into microfiber scaffolds for stable and reproducible 3D culture. When combined with full-length Biolaminin, it forms tissue-specific microenvironments that support long-term organoid formation and controlled differentiation. Biosilk 521 offers an optimal structure for hPSC integration, growth and lineage development.

What’s inside

• Protocols for creating attached and free-floating Biosilk and Biolaminin 3D scaffolds in 24- and 96-well formats
• Steps for preparing concentrated cell suspensions and combining them with Biosilk and Biolaminin without harming cell viability
• Guidance on generating dense foams, stabilizing the structure and supporting uniform 3D cell integration
• Recommended culture conditions for pluripotency maintenance and lineage-specific differentiation
• Instructions for lifting and transferring foams to low-attachment plates for long-term organoid culture
• Representative images showing foam formation, scaffold maturation and organoid morphology
• Troubleshooting tips for scaffold stability, bubble formation and uneven cell growth