Video: Passaging and culture of human PSC on Biolaminin 521

Resources – Video: Passaging and culture of human PSC on Biolaminin 521

Passaging and culture of human PSC on Biolaminin 521

This video describes how to culture human embryonic stem cells (hESC) and human-induced pluripotent stem cells (hiPSC) on human recombinant BioLaminin 521 substrates. The protocol can easily be made totally defined and animal component-free with your choice of culture medium since the BioLaminin 521 matrix works well in combination with most commercial media brands. Both enzyme and EDTA based dissociation can be used. Human pluripotent stem cells can routinely be cultured as single cells on BioLaminin 521 without need for ROCK inhibitor (ROCKi). 2018-05-08