Introduction to laminins
Stem cell culture on Biolaminin 521
Stem cell culturing is easy on human recombinant Biolaminin 521 LN (LN521). It facilitates self-renewal of both pluripotent human embryonic stem cells and induced pluripotent stem cells in a chemically defined, feeder-free and animal origin-free cell culture system.
LN521 allows the survival and expansion of human ES and iPS cells after plating from single-cell suspension. LN521 based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation. To culture, embryonic stem cells on LN521 enables easy and efficient expansion of your hESCs and iPSCs and provides a biorelevant in vitro stem cell niche.
Laminins are tissue-specific glycoproteins in the basement membrane, important for cell proliferation, migration and differentiation, and tissue development.
Laminin isoforms 521 and 511 are expressed by cells in the inner cell mass of the blastocyst and are therefore optimal as substrates for human pluripotent stem cells. Human recombinant laminin isoforms are developed for defined and animal origin-free stem cell culturing and primary cell cultures.
Derivation and culture of stem cells and primary cells on specific laminins
Professor Karl Tryggvason discusses the use of biologically relevant human recombinant laminins from BioLamina that successfully recreates specific cell niches in the cell culture dish. This enables robust stem cell culture methods that in practical solves all current technical challenges with culturing human ES and iPS cells. It also describes novel data for culturing primary cells such as cardiomyocytes, pancreatic beta cells, and endothelial cells.
Dr. Kristian Tryggvason tells the story about BioLamina and why biologically relevant laminins are the best matrix for cell culture, including the culture of stem cells and primary cells.