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What is the difference between culturing cells in Biosilk compared to a hydrogel?
There are big differences in the spreading and expansion of cells within Biosilk compared to when encapsulating cells in a hydrogel. […]
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Is it possible to functionalize Biosilk with other proteins than laminin?
Yes, most proteins will have a tendency to get entrapped/entangled in the Biosilk.
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How long does it take for the cells to degrade the silk molecules?
Approximately one to six months.
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Are the Biosilk products defined and animal origin-free?
The Biosilk products are defined and animal origin-free to the primary level (Ref: ISCT guidance document “ISCT Animal-Free Origin Survey Results-Summary”).
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Have you measured the stiffness of the Biosilk? At the minimal cell to Biosilk ratio, what is the stiffness?
Biosilk with integrated mesenchymal stem cells has a Youngs modulus 1.8 +/− 0.5 MPa with 25% elongation.
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What is the viscosity and melting point of Biosilk?
When Biosilk or Biosilk 521 is in liquid form, the viscosity is similar to PBS buffer (only 0.3wt% protein). When the Biosilk products […]
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What is the molecular weight of Biosilk?
25 kDa
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Do you have protocols for creating Biosilk sheets or films rather than foams?
Sheets of Biosilk can be formed by placing a solution of 1 mg/ml still for 8 h (unpublished data).Films/coatings of […]
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Is it possible to make different layers of Biosilk foam on top of each other?
Yes, but the first foam generated needs to be stabilized at least 20 min before adding the next layer.
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Does the Biosilk have some kind of autofluorescence?
Yes, you can expect some autofluorescence, at lower wavelengths, but it is not worse compared to tissue. A couple of collaborators have […]