Here is a selection of publications where different laminin isoforms were used to create more authentic cell culture systems.

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  • Properly formed but improperly localized synaptic specializations in the absence of laminin α4

    Bruce L. Patton, Jeanette M. Cunningham, Jill Thyboll, Jarkko Kortesmaa, Håkan Westerblad, Lars Edström, Karl Tryggvason & Joshua R. Sanes. Nature Neuroscience, 2001

    The authors show that the formation and localization of synaptic specializations are regulated separately, and α4β2γ1 (laminin-421) is critical in the latter process.

  • Integrin-laminin interactions controlling neurite outgrowth from adult DRG neurons in vitro

    Stefan Plantman, Manuel Patarroyo, Kaj Fried, Anna Domogatskaya, Karl Tryggvason, Henrik Hammarberg, Staffan Cullheim. Molecular and Cellular Neuroscience, 2008

    The authors examined neurite outgrowth from adult mouse DRG neurons on four laminin isoforms (laminin-1/LM-111, laminin-2/LM-211, laminin-8/LM-411 and laminin-10/LM-511) in vitro. The growth on LN111 and LN511 was trophic factor-independent and superior to the one on LN211 and LN411.

  • Laminins 2 (α2β1γ1, Lm-211) and 8 (α4β1γ1, Lm-411) are synthesized and secreted by tooth pulp fibroblasts and differentially promote neurite outgrowth from trigeminal ganglion sensory neurons

    Kaj Fried, Wondossen Sime, Christina Lillesaar, Ismo Virtanen, Karl Tryggvasson, Manuel Patarroyo. Experimental Cell Research, 2005

    The authors reported that LN-2 (LN211) and LN-8 (LN411) are synthesized by tooth pulp fibroblasts and differentially promote neurite outgrowth from TG neurons. They discuss that LN-8 may contribute to sensory innervation of teeth and other tissues during development and/or regeneration.

  • Impeded Interaction between Schwann Cells and Axons in the Absence of Laminin α4

    Wilhelm Wallquist, Stefan Plantman, Sebastian Thams, Jill Thyboll, Jarkko Kortesmaa, Jan Lännergren, Anna Domogatskaya, Sven Ove Ögren, Mårten Risling, Henrik Hammarberg, Karl Tryggvason and Staffan Cullheim. JNeurosci, 2005

    The Schwann cell basal lamina is is required for normal myelination. The authors show here that absence of the laminin α4 chain, which distinguishes laminin-411 from laminin-211, leads to a disturbance in radial sorting, impaired myelination, and signs of ataxia and proprioceptive disturbances. Laminin-211 and laminin-411 have different critical functions in peripheral nerves, mediated by different integrin receptors.

  • The hippocampal laminin matrix is dynamic and critical for neuronal survival

    Zu-Lin Chen, Justin A. Indyk, and Sidney Strickland Molecular Biology of the Cell, 2003

    In this article, the authors investigated how laminin is involved in neuronal viability by infusing laminin-1 (α1,β1,γ1) into the mouse hippocampus. The results demonstrate that the laminin matrix is a dynamic structure amenable to modification by exogenous molecules.

  • Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina

    Gnanaguru G., Bachay G., Biswas S., Pinzón-Duarte G., Hunter D.D. Brunken W.J.Development, 2013

    Astrocytes regulate retinal vascular development. Generated extrinsically to the retina, astrocytes migrate into the retina through the optic nerve head. In this study, we show that astrocytes migrate within a laminin-containing basement membrane. Genetic deletion of the laminin β2 and γ3 chains affects astrocyte migration and spatial distribution. We show that laminins act as haptotactic factors in vitro in an isoform-specific manner, inducing astrocyte migration and promoting astrocyte differentiation. The addition of exogenous laminins to laminin-null retinal explants rescues astrocyte migration and spatial patterning. Furthermore, we show that the loss of laminins reduces β1 integrin expression in astrocytes which can be restored when astrocytes are cultured on Biolaminin 521 or Matrigel. Finally, they show that laminins containing β2 and γ3 chains regulate subsequent retinal blood vessel growth and maintain vascular integrity. These in vivo and in vitro studies demonstrate clearly that laminins containing β2 and γ3 chains are indispensable for migration and spatial organization of astrocytes and that they play a crucial role during retinal angiogenesis in vivo.

  • Astrocytic laminin regulates pericyte differentiation and maintains blood-brain barrier integrity

    Yao Y., Chen Z-L., Norris E.H., Strickland S.Nature comm, 2014

    Here they show that lack of astrocytic laminin, a brain-specific BM component, induces BBB breakdown. Use conditional knockout mice and an acute adenovirus-mediated knockdown model. Using functional blocking antibody and RNAi, we further demonstrate that astrocytic laminin, by binding to integrin a2 receptor, prevents pericyte differentiation from the BBB-stabilizing resting stage to the BBB-disrupting contractile stage, and thus maintains the integrity of BBB. Loss of astrocytic laminin also decreases aquaporin-4 (AQP4) and tight junction protein expression. These results indicate that astrocytic laminin maintains the integrity of BBB through, at least in part, regulation of pericyte differentiation.

  • Ablation of astrocytic laminin impairs vascular smooth muscle cell function and leads to hemorrhagic stroke

    Chen Z-L., Yao Y., Norris E.H., Kruyer A., Jno-Charles O., Akhmerov A., Strickland S. J Cell Biol. 2013

    Astrocytes express laminin-111 and 211 and assemble basement membranes (BMs) at their endfeet. Here the authors show that ablation of astrocytic laminin disrupted endfeet BMs and led to hemorrhage in deep brain regions of adult mice. The lack of astrocytic laminin led to impaired function of vascular smooth muscle cells, fragmentation and vascular wall disassembly where astrocytes have a closer association with VSMCs in small arterioles. Acute disruption of astrocytic laminin in the striatum of adult mice also impaired vascular smooth muscle cells function, indicating that laminin is necessary for vascular smooth muscle cell maintenance. In vitro, both astrocytes and astrocytic laminin promoted brain vascular smooth muscle cell differentiation.

  • Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination.

    Ghidinelli M., Poitelon Y., Shin YK., Ameroso D., Williamson C., Ferri C., Pellegatta M., Espino K., Mogha A., Monk K., Podini P., Taveggia C., Nave K.A., Wrabetz L., Park H.T., Feltri M.L.PLoS Biology, 2017

    Ghidinelli and colleagues, through their in vivo and in vitro studies have clearly shown that Laminin 211, apart from promotion, can also inhibit myelination by modulating neuregulin 1 type III activity via PKA signaling and thereby prevent inappropriate and excessive myelin wrapping of small nerve fibers.

  • YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells

    Poitelon Y., Lopez-Anido C., Catignas K., Berti C., Palmisano M., Williamson C., Ameroso D., Abiko K., Hwang Y., Gregorieff A., Wrana JL., Asmani M., Zhao R., Sim FJ., Wrabetz L., Svaren J., Feltri ML. Nature Neuroscience, 2016

    A mechanistic article just published by Poitelon and colleagues in Nat Neurosci, adding further evidence for the importance of laminin-211 for radial sorting and proper axon myelination by Schwann cells. The authors show that laminin-211 in combination with mechanical stimuli activate and modulate Yap and Taz, which are downstream effectors in the Hippo pathway, required for radial sorting fo axons and subsequent myelination.