Biolaminin 521 LN (LN521)
Human recombinant laminin 521
Biolaminin 521 LN is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal origin-free stem cell culture system. Biolaminin 521 enables efficient single-cell passaging of genetically stable and pluripotent stem cells without the need for any apoptosis inhibitors for the superior quality of your cells and studies.
A biologically relevant culture environment
Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue-specific.
Biolaminin 521 LN (LN521-02 and LN521-05) is a full-length, human, recombinant laminin 521 substrate, the only one of its kind on the market, providing an optimal environment for feeder-free culture of hPSCs. Laminin 521 is a key cell adhesion protein of the natural stem cell niche, expressed and secreted by hPSCs in the inner cell mass of the embryo. The Biolaminin 521 substrate recreates a biologically relevant hPSC milieu in vitro, promoting attachment, high survival, and robust long-term self-renewal of hPSCs. In addition, the more authentic culture environment primes the pluripotent stem cells for more efficient differentiation, enhanced cell maturation, polarization, and organization of specialized cell types.
The laminin 521 cell culture substrates have a positive effect on stabilizing and homogenizing pluripotent gene expression profiles between hES cell lines, providing a more controllable and robust culture system compared to the feeder- and many other feeder-free culture systems. Via the interaction with relevant cell surface receptors, laminins activate cell signaling cascades (e.g. PI3K/Akt pathway), giving consistent cellular responses and improved cell functionality, ultimately saving researchers both time and cost.
After birth, α5-laminins represent the most common laminins in the body and LN521 supports many diverse tissue cell types, such as cells from pancreas, vascular, nervous and muscular systems.
Pluripotent stem cellsBiorelevant culture of human ES and iPS cells on Biolaminin substrates Biolaminin 521 successfully replicates the genuine human stem cell niche […]VIEW application
3D cultureBiolaminin and Biosilk Key Advantages 3D cell culture and organoid formation 3D culture systems for disease modeling, drug screening and […]VIEW application
Clonal stem cell cultureBiorelevant clonal stem cell culture on Biolaminin substrates Biolaminin 521 successfully replicates the genuine human stem cell niche in vitro […]VIEW application
iPSC reprogramming and expansionReprogramming and expansion of human iPS cells on Biolaminin substrates High-efficiency iPSC reprogramming and expansion on Biolaminin 521 Human recombinant Biolaminin […]VIEW application
Hematopoietic stem cells and blood cellsBiorelevant culture of hematopoietic cells Laminins play a major role in the hematopoietic microenvironment Laminins are widely expressed in human bone […]VIEW application
Neural cellsBiorelevant culture of neural cells on Biolaminin substrates Laminins are widely expressed in the nervous system and are essential for […]VIEW application
Hepatocytes and hepatoblastsBiorelevant culture of liver cells on Biolaminin substrates Several laminins play a vital role in liver progenitor cell-mediated regeneration The […]VIEW application
Eye cellsBiorelevant culture of eye cells Laminin proteins are important components in the eye microenvironment Biolaminin products have been succesfully used […]VIEW application
Endothelial cellsBiorelevant culture of endothelial cells on Biolaminin substrates Laminin-521 can be used as an optimal matrix for primary vascular endothelial […]VIEW application
Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
Rodin S., Antonsson L., Niaudet C., Simonson O.E., Salmela E., Hansson E.M., Domogatskaya A., Xiao Z., Damdimopoulou P., Sheikhi M., Inzunza J., Nilsson A.S., Baker D., Kuiper R., Sun Y., Blennow E., Nordenskjöld M., Grinnemo K.H., Kere J., Betsholtz C., Hovatta O., Tryggvason K.
Nature Communications, 2014
Monolayer culturing and cloning of human pluripotent stem cells on laminin-521 based matrices under xeno-free and chemically defined conditions
Rodin S., Antonsson L., Hovatta O., Tryggvason K.
Nature Protocols, 2014
Laperle A., Hsiao C., Lampe M., Mortier J., Saha K., Palecek S.P., and Masters K.S.
Stem Cell Reports, 2015
A deﬁned xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-speciﬁc induced pluripotent stem cells
Hong Fang Lu, Chou Chai, Tze Chiun Lim, Meng Fatt Leong, Jia Kai Lim, Shujun Gao, Kah Leong Lim, Andrew C.A. Wan.
Laminin 521 stabilizes the pluripotency expression pattern of human embryonic stem cells initially derived on feeder cells
Albalushi H., Kurek M., Karlsson L., Landreh L., Rós Kjartansdóttir K., Söder O., Hovatta O., Stukenborg J-B.
Stem Cell International, 2017
Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP
Zhang D., Yang S., Toledo E.M., Gyllborg D., Saltó C., Villaescusa J.C., Arenas E.
Sci Signal. 2017
Takamichi Miyazaki, Norio Nakatsuji & Hirofumi Suemori.
Bergström R, Ström S, Holm F, Feki A, Hovatta O
Methods Mol Biol, 2011
Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.