Our technology

A biorelevant cell culture system for robust expansion, maintenance, and subsequent differentiation of human primary cell lines.

Biolaminin key advantages

Full-length laminins are essential for cells in the body; therefore, they make a real difference in the cell functionality of ex vivo cell cultures. Biolaminin products offer a biologically relevant matrix that mimics the authentic ECM-cell interaction.

Biolaminin products are fully defined and batch-to-batch consistent enabling reproducible results and credible conclusions. The products give uniform pluripotency gene expression patterns between cell lines and reduce experimental variability.

Switching to biorelevant Biolaminin in your cell culture system results in more efficient differentiation, cell maturation, and organization of specialized cell types, such as hepatocytes, cardiomyocytes, and neurons.

Biolaminin products are scientifically proven to increase cell proliferation rate with stable phenotype and facilitate the use of low passage cell lines for the production of clinically relevant cell numbers of differentiated cell types.

The products are defined and xeno-free, making them ideal components for basic research and all the way to clinical applications (Cell Therapy Grade). Certificate of Analysis, Animal Origin Free statement, and Material Safety Data Sheet are available for all our products.

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BioLamina matrices enable scientific progress

Whether for disease modeling or preparing cells for clinical use, you need a cell culture system that mimics the in vivo environment to achieve high-quality cell products. This is where biorelevant, full-length Biolaminin® proteins come into play. Laminins are important extracellular matrix proteins that surround cells in the body. The effectiveness of the Biolaminin isoforms has been shown in a multitude of publications: take a look at these scientific innovations by our customers.

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Laminin Biology

Laminins are key proteins in the basement membrane

Laminins are a large family of conserved, multidomain glycoproteins (at least 16 isoforms in mammals) that form part of the extracellular matrix. They are a major component of the basal lamina, the protein network supporting most cells and tissues. Laminins are critical for tissue function and survival, and many isoforms show distinct cell-type specificity. Without the right laminin combination, cells and tissues become dysfunctional.

Each laminin isoform is composed of three interwoven chains—α, β, and γ. Five distinct α variants, four β variants, and three γ variants can combine in different ways. For example, laminin 521 (LN521) is made of α5, β2, and γ1 chains. The resulting trimer forms a cross-like structure that can bind both extracellular matrix molecules and various cell membrane receptors.

Tissue-specific culture substrates for improved cell functionality

In the body, cells live in specialized microenvironments—or niches—that protect and sustain them. Laminin 511 and 521 (which both have an α5 chain) are key adhesion proteins in the blastocyst stem cell niche, produced by embryonic stem cells in the embryo’s inner cell mass. Biolaminin 521 recreates this environment in vitro for human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is critical for their survival and self-renewal through autocrine and paracrine signaling. Tissue-specific laminins can also support efficient differentiation of various specialized cell types, such as hepatocytes, skeletal muscle cells, and neural cells.

Only intact, full-length laminin replicates the biologically relevant conditions

Full-length laminins carry many binding sites for cell receptors and extracellular matrix factors. They can co-signal with growth factors and also buffer endogenously produced factors. By regulating cell anchorage, survival, proliferation, migration, organization, and specialization, laminins directly enhance cell functionality.

The crucial extracellular microenvironment

Stem cells are guided by soluble factors, ECM proteins, and signals from neighboring cells. These signals engage both integrin and non-integrin receptors, triggering complex intracellular pathways that affect survival, self-renewal, migration, morphogenesis, and differentiation.

Full-length laminins for authentic cell culture from basic research to clinical applications

BioLamina offers Biolaminin®, a broad range of defined, animal origin–free, full-length human recombinant laminin substrates for 2D and 3D applications. They support reliable expansion of hESCs, iPSCs, MSCs, and the differentiation and maintenance of cells like hepatocytes, pancreatic cells, and various neural cells. Biolaminin substrates mimic natural cell–matrix interactions, leading to consistent cell responses and better functionality.

Biolaminin substrates are the only full-length, recombinant laminins on the market. Fragmented laminins or those isolated from tissue lack crucial domains. Only intact laminins provide an authentic culture environment, ensuring the proper extracellular network and correct cellular signaling.

Our Biolaminin matrices help you move from reproducible research to cell therapy development. They meet strict regulatory standards and support the scale-up of cell production while improving cell models.

  • Biolaminin LN is designed for reserch
  • Biolaminin MX is designed for translational research
  • Biolaminin CTG is designed for clinical applications

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WHAT OUR CUSTOMERS SAY

“We struggled for a number of years to establish a GMP manufacturing process for generating DA neurons from human embryonic stem cells in order to develop a stem cell-based therapy for Parkinson’s Disease. The recombinant laminins from BioLamina really made a world of difference! We now routinely use LN521 and EDTA for passaging the pluripotent stem cells and we have greater efficiency than ever before.”

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Introducing Biosilk™: a defined biomaterial for 3D primary cell culture and organoid formation

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Read more about biorelevant 3D culture

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