Biolaminin 332 LN (LN332)
Human recombinant laminin 332
Biolaminin 332 is a full-length and animal-origin free human laminin 332 product, which supports cells in epithelial basement membranes in several tissues.
A biologically relevant culture environment
Laminin 332 highly influences keratinocyte proliferation and mediates a strong adhesion of keratinocytes to the basement membrane through hemidesmosome protein complex (Ghohestani, 2001). Laminin 332 is highly enriched in epithelial basement membranes.
The importance of laminin 332 mediated adhesion in the skin is also evident in the human blistering disease Junctional Epidermolysis Bullosa, which is associated with laminin-332 gene mutations (DiPersio, 1997).
The expression of laminin 332 is upregulated during wound healing and has important effects on keratinocyte migration, invasion and eventually tissue remodeling (D’Alessio, 2008).
Laminin 332 is highly expressed in the catagen phase by keratinocytes in the basement membrane zone of hair follicles. It antagonizes laminin 511 driven hair growth in vitro of human hair follicles and, thus, hair growth has been suggested to be controlled by the relative expression of laminin 511 and laminin 332 (Conti, 2003; Sugawara, 2007; Li, 2003).
Structural changes in the RPE basement/Bruch’s membrane (BrM) lead to alterations in the RPE-matrix adhesion which is commonly the cause for retinal diseases (Zarbin, 2004).
Laminins are produced by and secreted from RPE cells themselves into the RPE basement membrane (Campochiaro, 1986). In particular, the RPE cells produce and bind the strongest to laminin 332 and in vitro, laminin 332 preserves phenotypic stability of mature RPE cells the best (Aisenbrey, 2006).
Recommended applications
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Cancer cells
Biorelevant culture of cancer stem cells on Biolaminin substrates Cancer cells use laminin/integrin signaling pathways that promote tumor cell growth, invasion, and […]View application -
Bone and cartilage cells
Biorelevant culture of bone and cartilage cells on Biolaminin substrates High expression of laminin isoforms in the bone microenvironment Bone cells, including include […]View application -
Skin cells
Biorelevant culture of keratinocytes on Biolaminin substrates The basement membrane composition in the epidermis Human epidermal keratinocyte (HEK) cells are […]View application -
Hair follicle cells
Biorelevant culture of hair follicle stem cells on Biolaminin substrates Epithelial tissues contain a-5 and a-3 laminin isoforms Epithelial tissues are […]View application -
Mesenchymal stem cells
Biorelevant culture of human mesenchymal stem cells on Biolaminin substrates Laminin 521 is widely expressed in the bone marrow and is an […]View application
Key features
Coating plates
1. Slowly thaw recombinant laminins at +4°C before use.
2. Dilute the thawed laminin stock solution with 1xDPBS containing Ca2+ and Mg2+.
3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 μg/cm2. The optimal coating concentration is cell line-dependent.
4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.
Important notes
- The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.
- Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.
- Avoid long exposure of the protein to ambient temperatures.
- For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.
- The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.
- Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.
Product name
Biolaminin 332 LN
Product code
LN332-0202
LN332-0502
Declaration
For research use only
Storage
-20°C to -80°C
Concentration
0.1 mg/ml
Appearance
Clear, colorless, buffered solution with a
pH of 7.2 with 10% glycerol and 0.02% NaN3
Shipping condition
Dry Ice
Protein name
Laminin 332 (Laminin-5)
Classification
Animal origin-free, human recombinant protein
Product application
Culture of keratinocytes, RPE cells, and hair follicle stem cells
Size guide
Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.
Please see the coating instructions for concentration and volume guidelines.