Biolaminin 511 LN (LN511)

Important notes

Preparation and handling

• Perform all procedures under sterile conditions using aseptic techniques.
• Minimize exposure of the protein to ambient temperatures.

Storage and stability

Laminin stock solution storage

• Store the frozen laminin stock solution at -20°C to -80°C for long-term stability. Refer to the product-specific Certificate of Analysis (CoA) for detailed shelf-life information.
• Avoid repeated freeze-thaw cycles.
• For long-term storage of thawed stock solution, dispense into working aliquots and store at -30°C to -80°C. Thawed, undiluted Biolaminin stock remains stable for 3 months at +2°C to +8°C.

Coated plate storage

• Store coated plates aseptically at +2°C to +8°C for up to 4 weeks. Do not let the surface dry.

Cell culture setup

• Use appropriate culture media and dissociation reagents to create fully defined, animal component-free protocols.
• Ensure high-quality cells when transferring to the LN511 matrix.
• When moving your cells from another feeder-free matrix (e.g., Matrigel), we recommend starting with a smaller well format (e.g., 96-well or 48-well format) and a higher seeding density for the first few passages. This allows the cells to adapt to the laminin matrix before increasing the culture well format and lowering the seeding density.

Troubleshooting Guide

Biolaminin plate coating

Uneven cell spread is often a coating issue and may be caused by:

• Low coating concentration: Ensure the laminin coating concentration is high enough to support even cell growth. Increase the concentration if necessary.

• Poor coating coverage or plate drying: Confirm that the entire surface is covered with the laminin coating solution when preparing fresh plates. Avoid drying out the plate, as this will inactivate the laminin. Prolonged time in the incubator or long storage without proper sealing can cause evaporation, leading to localized drying, often in the center of the plate.