Biosilk 521

In the Biosilk fibrillar network, oxygen, nutrients, and patterning factors can flow into the inner part of the organoid. Cells are not encapsulated. This enables long-term differentiation protocols and makes it possible to generate larger organoids with uniform cellular specialization and organization, without an increased risk of getting necrotic centers.

Biosilk is a completely defined recombinant spider silk protein matrix that is non-immunogenic and biodegradable, further facilitating its use in clinical applications.

All our matrices are chemically defined and animal origin-free, which makes them ideal substrates for each level of the scientific process – from basic research to clinical applications.

Biosilk can self-assemble into a microfibrous network, such as foams. Due to its favorable functional and mechanical properties, Biosilk can support extensive cellular remodeling, self-organization, and morphogenesis.

3D models are pursued to reproduce the way cells grow and interact with each other and the microenvironment in native tissues. To provide proper microenvironmental contacts and support correct cell phenotype, Biolaminin isoforms can be added into different organoid culture systems. Especially, the Biosilk 3D network can be easily biofunctionalized with Biolaminin isoforms and other extracellular matrix proteins. This results in a more tissue-like microenvironment in which the integrin-involved attachment to the Biosilk fibers gives the cells an elongated shape, with organized cytoskeletons and the formation of defined focal adhesion points. Increased biological relevance increases the chances of capturing robust and applicable experimental and pre-clinical results.