The matrix to trust for your stem cell therapy success
Successful cell therapy requires reproducibility, global regulatory compliance, and most importantly, the generation of truly functional clinical cells. Biolaminin® excels in supporting all these aspects.
Key advantages
Key advantages of Biolaminin in stem cell therapy production
Scientifically proven matrix to streamline differentiation, stabilize identity, and enhance cell growth across various culture platforms and conditions.
Reproducible and stable process
➞ Achieve consistently functional cell products— reliable every time
➞ Maintain stem cell pluripotency and cell identity
Reproducible and stable process
Enhanced cell functionality and robust differentiation
Numerous studies show improved cell maturation and ease of primary cell culture on tissue-specific Biolaminin proteins. This includes PSC differentiation into neurons, hepatocytes, pancreatic islets, vascular endothelial cells, and corneal cells. These defined and biologically relevant matrices help consistently achieve the desired end product. See these examples:
Higher reproducibility and maturity of ovarian support cells to improve oocyte in vitro maturation for fertility treatments
Gameto’s Fertilo in vitro maturation (IVM) asset represents a significant advancement for in vitro fertility (IVF) treatments. Ovarian support cells (OSCs) differentiated on full-length laminin-521 exhibited a more mature granulosa-like phenotype and demonstrated significantly improved reproducibility compared to Matrigel, Vitronectin, or truncated laminin-511.
Read more: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment. Paulsen et al. 2024 bioRXiv
Consistent reproducibility in a laminin-based differentiation protocol for regenerative cardiology
Employing LN221, a laminin abundant in the heart, the scientists established a stable protocol for differentiating human embryonic stem cells (hESCs) into cardiovascular progenitors (CVPs). This method results in upregulating markers associated with cardiac development while downregulating tumor-inducing markers. The laminin-based differentiation enables high reproducibility between different hESC lines. These differentiated cells also exhibit enhanced heart function with no signs of tumorigenicity observed following cell transplantation in pigs.
Read more: In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors. Yap et al. 2019 Cell Reports
Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs. Yap et al. 2023 NPJ Regen Med
Stem cell-derived hepatocytes with significantly increased metabolic function, more organized structures, and a decrease in unwanted gene expression
Read more: Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes. Cameron et al. 2015 Stem Cell Reports.
Highest maturation of myotubes on laminin isoforms
Laminin matrix enables the cultivation of primary myotubes, reproducibly promoting the formation of acetylcholine receptor (AChR) clusters from cryopreserved patient muscle biopsies.
Actin-rich podosomes. Laminin-221 and -211 together supported the highest maturation of AChR clusters in C2C12-myotubes.
Read more: An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery. Pęziński et al. 2020 Sci Rep 10, 4524 (2020)
Maintained stem cell pluripotency
Human ES and iPS cells remain pluripotent on Biolaminin 521
hES (HS181) and hiPS (iPSC3) cells cultured on Biolaminin 521 CTG (Cell Therapy Grade) remain pluripotent, confirmed here by immunocytochemistry for OCT4 and NANOG after 10 days of culture.
hPSCs demonstrate multipotency with embryoid body (EB) formation after long-term culture on Biolaminin 521
hES (HS181) cells cultured on Biolaminin 521 CT maintain the protein pluripotent markers NANOG and OCT4 when cultured as single cells for 10 passages (A). Upon EB formation, the pluripotent marker (B) protein expression decreases, and ectoderm (C, PAX6) and mesoderm marker expressions (C, BRACHYURY) are upregulated.
Maintenance of normal karyotype in hPSCs
hES cells present a normal karyotype after 13 passages on Biolaminin 521 CT.
Read more:
- Homogenized gene expression across ESC lines: Laminin 521 stabilizes the pluripotency expression pattern of human embryonic stem cells initially derived on feeder cells. Albalushi et al. Stem Cell International, 2017
- A GMP-grade protocol for generating astrocytes from hESCs: Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Izrael et al. Stem Cell R&T, 2018
- Transplanting neuroepithelial-like stem cells derived from GMP-compliant PSCs: Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia. Xu et al. eBioMedicine 2022
Maximized cell
yield
➞ Enhance cell proliferation and survival
➞ Optimize utilization of culture surface area
Maximized cell yield
Biolaminin 521 outperforms other matrix types
Human pluripotent stem cells passaged as single cells (without ROCKi) on Biolaminin 521 CT and LN accumulate significantly faster than on other tested matrix types. Fibronectin substrates failed to support cell growth. Additionally, Biolaminin 521 outperforms Matrigel (an EHS extract) and Vitronectin in cell numbers, in addition to offering a defined and biologically relevant option.
Cells recover instantly after single-cell seeding – 2-7 times more cells over 10 days
The full-length Biolaminin 521 supports significantly enhanced proliferation compared to fragmented laminins, with 2-7 times more pluripotent stem cells over 10 days. With full-length laminin, cells survive single-cell passaging without ROCKi, reaching confluency already at day 3 post-seeding.
Full-length laminin enhances growth on the entire surface area by enabling cell migration
Cells migrate swiftly on Biolaminin 521, reaching 100% closure of surface area after 6-7 days, whereas cells on fragmented laminins only reach a max of 54%.
Read more:
- Over 40-fold neural progenitor cell expansion with laminin-521: Predictive Markers Guide Differentiation to Improve Graft Outcome in Clinical Translation of hESC-Based Therapy for Parkinson’s Disease. Kirkeby et al. 2016 Cell Stem Cell
- The highest hepatic progenitor proliferation with laminin-521: p53 positively regulates the proliferation of hepatic progenitor cells promoted by laminin-521. Ma et al. 2022 Sig Transduct Target Ther
- 10x more hiPSC population doublings on laminin-521: Laminin as a Potent Substrate for Large-Scale Expansion of Human Induced Pluripotent Stem Cells in a Closed Cell Expansion System. Mesquita et al. 2019 Stem Cells International
Wide platform compatibility
➞ From single cells to large-scale expansion; applicable to various protocols with minimal adjustment
➞ From concept to standardized protocols and global regulatory compliance
Wide platform compatibility
The Biolaminin matrix integrates into every stage of a PSC-based therapy production processes
✔ From single cells on microtiter plates to stable expansion in bioreactors
✔ In automation systems and imaging
✔ Without apoptosis inhibitor supplements
✔ Independent of media
✔ Across various surfaces from plastic to glass
✔ From primary cell culture, reprogramming, gene editing, and clone expansion to maturation
Significantly increased single-cell survival and transfection efficiency
Switching the matrix to Biolaminin 521 for the whole cloning process increased single-cell survival from 36 % to 70 %, having the most significant impact during the gradual optimization of the xeno-free cloning of CRISPed iPSCs. The high cell survival was accompanied by a high transfection efficiency of more than 97 %.
Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling. Namipashaki et al. 2023 Stem Cell Reports.
Biolaminin 521 increases single-cell cloning efficiency and imaging quality in automated systems
Three different automated cell isolation and dispensing devices were described to enhance the single-cell cloning process for hPSCs after CRISPR/Cas9-mediated genome editing. Biolaminin 521 significantly increased single-cell cloning efficiency compared to commonly used ECMs such as Matrigel or Geltrex.
In automated workflows, the purity of Biolaminin enhances imaging, facilitating high-throughput hPSC clonal selection and expansion.
Read more: Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells. Vallone et al. 2020 Curr Protoc Stem Cell Biol.
Enhanced attachment, spreading, and phenotype on large-scale systems coated with Biolaminin
Biolaminin 521 coating of the porous hollow fibers in the Quantum Expansion System significantly increased cell numbers. The cells maintained pluripotency, karyotype, and differentiation capability to all three germ layers.
Read more: Laminin as a Potent Substrate for Large-Scale Expansion of Human Induced Pluripotent Stem Cells in a Closed Cell Expansion System. Mesquita et al. 2019 Stem Cells International
Defined, xeno-free, and scalable culture system using Biolaminin 521 coating on polystyrene microcarriers, resulting in cost savings
Biolaminin 521 induced more efficient hiPSC and hESC attachment (87%) and spreading (85%), resulting in 34% of overall matrix and material cost savings.
Read more: Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures. Lam et al. 2015. Bioresearch Open Access.
Supporting your clinical needs
Robust quality documentation and comprehensive support
Several cell therapy programs with Biolaminin have already been acknowledged by regulatory authorities across the globe, including the FDA. The formulation and production process of Biolaminin CT are devoid of animal-derived components up to the secondary level. We prioritize maintaining meticulous traceability of processes and raw materials to meet the rigorous standards necessary for the production of cell-based Advanced Therapy Medicinal Products (ATMP). We also provide scientific and quality-related support to aid you in your cell culture process with our matrices.
Generating GMP-compliant cells
A compelling example of the feasibility of producing GMP-grade cell products was recently described. Biolaminin 521 matrix supported superior colony outgrowth during reprogramming to clinical-grade hiPSCs. The resulting master cell banks were shown to differentiate into clinical products, such as cardiomyocytes, for regenerative medicine applications.
Read more: Good Manufacturing Practice–compliant human induced pluripotent stem cells: from bench to putative clinical products. Novoa et al. 2024 Cytotherapy.
“We have struggled for a number of years to establish a GMP manufacturing process for generating DA neurons from human embryonic stem cells in order to develop a stem cell-based therapy for Parkinson’s Disease.
The recombinant laminins from BioLamina really made a world of difference!”
Prof. Malin Parmar
Lund University
What makes us unique
What makes us unique—biology is the key
✓ The in vivo stem cell matrix
The key to vastly improved cell function lies in the essential roles of laminins within the stem cell microenvironment and across human tissues.
In the early embryo, laminin-521 is the primary extracellular matrix (ECM) molecule to appear within the embryonic stem cell niche, already at the four-cell stage. Unlike other matrix products, full-length laminin serves as the foundational support for self-renewing stem cells in their natural niche.
✓ The only full-length laminin for authentic cell signaling
Laminin functions as a crucial link between the extracellular matrix (ECM) and cells, actively directing cell identity, proliferation, and survival through the activation of core signaling pathways.
Unlike fragmented laminin products, full-length laminin can bind with all relevant cell surface receptors, including integrins, dystroglycans, and syndecans. Importantly, it is also able to interact with various microenvironmental factors, including growth factors.
✓ Tissue-specific laminin isoforms for specialized cell function
Laminin isoforms display diverse compositions across tissues, supporting cell specialization and functionality in a tissue-specific manner. Our exclusively full-length laminin products provide the largest laminin isoform portfolio on the market.
Our products
Take your stem cell therapy program from concept to clinic
Enhance your therapeutic cell production process with Biolaminin MX with a seamless transition to the cell therapy grade CTG product.
Explore our products
Optimize your differentiation process
Explore our tissue-specific laminin isoforms – tailored to your differentiation needs!
Read more about Biolaminin matrices and their applications
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Application note 015: Cell therapy grade Biolaminin 521 CTG
Features of the Biolaminin CTG product designed for clinical use
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Product sheet: Biolaminin 521 cell culture substrates
See how our CT, and MX products differ
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Application note 022: BioLamina products in research and manufacturing – Biolaminin® matrices for all steps and scales
Clinically adaptable Biolaminin cell adhesion coating for higher yield in bioreactor systems
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Application note 018: Large-scale cell production
Clinically adaptable Biolaminin cell adhesion coating for higher yield in bioreactor systems
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Application note 020: Efficient gene editing on Biolaminin® 521 substrate
Efficient gene editing on Biolaminin® 521 substrate
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Application overview 008: Full-length laminin proteins are crucial for stem cells
Full-length laminins are crucial components in creating the stem cell niche in vivo and in vitro
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