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Application note 009-02: Cardiomyocytes differentiation — on combination of full-length Biolaminin® 521 (LN521) and Biolaminin 221 (LN221) substrates)
Pluripotent stem cell (PSC)-derived cardiomyocytes play a key role in therapies of the future, serving directly as cellular therapy, as source material for tissue-specific extracellular vesicles, and as a platform for drug development. This requires standardized, reproducible, and efficient derivation of cardiomyocytes.This application note describes a published method on how to derive xeno-free and defined cardiovascular progenitor (CVP) cells and cardiomyocytes (CM) at high efficiency with a combination of Biolaminin 521 and 221 substrates (Yap L. et al. 2019 and 2023). The method is twice as efficient as other standardized substrates. The cells mature and display critical functional features, such as contraction and electrophysiological signatures, improving heart function over time after successful integration into infarcted pig heart.
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Reproducible differentiation of functional PSC-derived cardiomyocytes on heart-abundant laminin substrates
Scientific Spotlight The easiest path to stable cardiomyocyte differentiation Learn how Dr. Lynn Yap and colleagues at Duke NUS Singapore […]
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Pluripotent stem cell-derived cardiovascular progenitors differentiated on laminin 221 regenerate and improve function of infarcted swine hearts
Pluripotent stem cell-derived cardiovascular progenitors differentiated on laminin 221 regenerate and improve function of infarcted swine hearts Lynn Yap, Li Yen Chong, Clarissa Tan, Swarnaseetha Adusumalli, Millie Seow, Jing Guo, Zuhua Cai, Sze Jie Loo, Eric Lim, Narayan Lath, Lei Ye, Enrico Petretto, Karl Tryggvason […]
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In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors
In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors Yap L., Wang J.W., Moreno-Moral A., de Kleijn […]
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A Chemical Probe that Labels Human Pluripotent Stem Cells
A Chemical Probe that Labels Human Pluripotent Stem Cells Hirata N., Nakagawa M., Fujibayashi Y., Yamauchi K., Murata A., Minami […]
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Chemically defined generation of human cardiomyocytes
Chemically defined generation of human cardiomyocytes Burridge P., Elena Matsa E., Shukla P., Lin Z., Churko J., Ebert A., Lan […]
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Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface
Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface Sung T.-C., Su H.C, Ling Q.-D., […]
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A Small Molecule that Promotes Cardiac Differentiation of Human Pluripotent Stem Cells under Defined, Cytokine- and Xeno-free Conditions
A Small Molecule that Promotes Cardiac Differentiation of Human Pluripotent Stem Cells under Defined, Cytokine- and Xeno-free Conditions Minami I., […]
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Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells
Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells […]
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iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium
iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium Ja K.P., Miao Q., […]