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Application note 024: Biofunctionalized hydrogels — Biolaminin® proteins for tissue-specific functionalization
This application note introduces how recombinant full-length laminins, Biolaminin® can be used to create tissue-specific hydrogels of various types, from natural to synthetic and for R&D or clinical translation. The biofunctionalization of the various hydrogels improve
cell survival, attachment and differentiation of
PSC-derived cells of the eye, liver, cardiac and vascular
endothelium. The biofunctionalization also improved
cell type specific response, such as increased barrier
formation, metabolic activity.
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Application note 009: Cardiomyocyte differentiation — on a combination of full-length Biolaminin® 521 (LN521) and Biolaminin 221 (LN221) substrates
Pluripotent stem cell (PSC)-derived cardiomyocytes play a key role in therapies of the future, serving directly as cellular therapy, as source material for tissue-specific extracellular vesicles, and as a platform for drug development. This requires standardized, reproducible, and efficient derivation of cardiomyocytes.This application note describes a published method on how to derive xeno-free and defined cardiovascular progenitor (CVP) cells and cardiomyocytes (CM) at high efficiency with a combination of Biolaminin 521 and 221 substrates (Yap L. et al. 2019 and 2023). The method is twice as efficient as other standardized substrates. The cells mature and display critical functional features, such as contraction and electrophysiological signatures, improving heart function over time after successful integration into infarcted pig heart.
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Application note 004: Expansion of pluripotent stem cells on Biolaminin 521
Why to use Biolaminin for stem cell culture
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Application overview 008: Full-length laminin proteins are crucial for stem cells
Full-length laminins are crucial components in creating the stem cell niche in vivo and in vitro
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Application note 010: Hepatocyte differentiation on Biolaminin substrates
Full-length laminins support stem cell-derived differentiation and
primary hepatocyte culture
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Instructions 004: Neural crest differentiation from hPSCs on Biolaminin® 521
Protocol for neural crest cell differentiation
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Application note 021: Neural Crest differentiation on Biolaminin® 521 substrate
Generation of hPSC-derived neural crest cells on Biolaminin 521
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Application note 023: Next generation xeno-free and defined skin cell culture – on Biolaminin® 521 (LN521) and Biolaminin 511 (LN511) substrates
Skin is the largest organ of the body and our first line of defense against pathogens and environmental impacts. The skin is composed of two main layers, epidermis and dermis. They are separated by an ultrathin basement membrane (BM), highly enriched in laminin proteins, particularly the isoforms 332, 331, 521 and 511. Laminin 332/331 are mostly associated with adhesion and maturation and 521/511 with adhesion and proliferation. The complex interaction of BM and cells is essential for healthy skin development and tissue homeostasis, and essential part of the stem cell niche of the skin.
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Instructions 012: Biosilk® and Biolaminin® free-floating scaffolds in 96-well plate for hPSC culture
Guideline for human Pluripotent Stem Sell integration into free-floating Biosilk scaffolds fitted into 96-well plates.
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Application note 022: BioLamina products in research and manufacturing – Biolaminin® matrices for all steps and scales
Clinically adaptable Biolaminin cell adhesion coating for higher yield in bioreactor systems